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M94A2742.TXT
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Document 2742
DOCN M94A2742
TI Comparison of the sensitivity and specificity of acid dissociated and
regular HIV p24 antigen assays.
DT 9412
AU Hofheinz D; Toedter G; Coulter Immunology, Hialeah, FL 33010.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):235 (abstract no. PB0371). Unique
Identifier : AIDSLINE ICA10/94369833
AB OBJECTIVE: To compare individual results obtained with regular HIV-1 p24
(Abbott and Coulter), with those of acid dissociated HIV p24 (ICD-Prep).
METHODS: Serum or plasma from 778 HIV seropositive individuals and 258
HIV seronegative individuals collected retrospectively. All samples were
assayed following the Coulter HIV-1 p24 Antigen Assay with and without
the ICD-Prep procedure. Additionally, 499 HIV seropositive, HIV antigen
positive (Abbott, HIVAG-1) samples were run using the Coulter HIV p24
ICD-Prep procedure. All positive samples were repeated and neutralized
following manufactures recommended procedures. RESULTS: Of the 778
seropositive samples tested, 484 (62.2%) were positive by both Coulter
HIV p24 and ICD HIV p24. 175 (22.5%) were positive by ICD only, and 1
(0.1%) was positive by regular HIV p24 only. The remaining 118 (15.2%)
were negative by both procedures. With the ICD-Prep procedure, 496 of
499 (99.4%) HIVAG-1 positive samples were positive. DISCUSSION AND
CONCLUSION: When performed in a blinded study, 484 of 485 (99.8%) of
samples positive by regular HIV p24 are positive also by the ICD-Prep
method. When run on 499 known antigen positive samples, 99.4% (496) were
ICD positive. The four regular positive by ICD were all low positive. We
conclude that with the sensitivity and specificity of the ICD-Prep
procedure, it is not necessary to run the regular HIV p24 assay on sera.
DE AIDS Serodiagnosis/METHODS Comparative Study Human HIV Core Protein
p24/*BLOOD HIV Seronegativity HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
HIV-1/*IMMUNOLOGY Predictive Value of Tests Retrospective Studies
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).